They warned that homosexuals continue to be the target of attacks in the Dutch capital, once known as the 'Gay Capital' of Europe.
The demonstration was organised by rights group Right to Feel Safe.
The rally began on the city's Dam square before proceeding along places where gay people have recently been harassed or assaulted.
Posters detailing the incidents were affixed at each location.
Amsterdam Mayor Eberhard van der Laan recently called for measures to end homophobic aggression and violence.
Overall the day's event was peaceful where drag queens, lesbians, bisexual activists, androgynous artists and some transgendered rights supporters peacefully protested, sang songs, gave speeches (see video below), marched and called for tolerance and no violence towards LGBT people.